成果信息
Effective enhancement of resistance to Phytophthora infestans by overexpression of miR172a and b in Solanum lycopersicum
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在榜期数::ESI高被引论文(2018年第4期);
文献类型:期刊论文
标题:Effective enhancement of resistance to Phytophthora infestans by overexpression of miR172a and b in Solanum lycopersicum
作者:Luan, Yushi[1];Cui, Jun[1];Li, Jie[1];Jiang, Ning[1];Liu, Ping[1];Meng, Jun[2]
机构:
通讯作者:Meng, J (reprint author), Dalian Univ Technol, Sch Comp Sci & Technol, Dalian 116024, Peoples R China.
来源信息:年:2018  卷:247  期:1  页码范围:127-138  
期刊信息:PLANTA影响因子和分区  ISSN:0032-0935
增刊:正刊
学科:植物学和动物学
收录情况:SCIE(WOS:000419584800008)  PubMed(28884358)  Scopus  
所属部门:电子信息与电气工程学部;化工与环境生命学部
重要成果类型:ESI高被引
语言:外文
链接地址:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85028960193&doi=10.1007%2fs00425-017-2773-x&partnerID=40&md5=86e3c6e56dceafd8cb4fff5d562242ab
发表时间:2018-01-01
全文链接:DOI百度学术
被引频次:4
人气指数:659
浏览次数:659
基金:National Natural Science Foundation of China [31471880, 61472061]
关键词:ERF gene; Late blight; MiR172; Resistance; Transgenic tomato
摘要:Overexpression of miR172a and b in tomato ( Solanum lycopersicum ) Zaofen No. 2 increased resistance to Phytophthora infestans infection by suppressing of an AP2/ERF transcription factor. The miR172 family has been shown to participate in the growth phase transition, flowering time control, abiotic and biotic stresses by regulating the expression of a small group of AP2/ERF transcription factors. In this study, the precursors of miR172a and b were cloned from tomato, Solanum pimpinellifolium L3708. We used the degradome sequencing to determine the cleavage site of miR172 to a member of the AP2/ERF transcription factor family (Solyc11g072600.1.1). qRT-PCR results showed that the expression of AP2/ERF was negatively correlated with the expression of miR172 in S. pimpinellifolium L3708 infected with Phytophthora infestans. Overexpression of miR172a and b in S. lycopersicum Zaofen No. 2 conferred greater resistance to P. infestans infection, as evidenced by decreased disease index, lesion sizes, and P. infestans abundance. The SOD and POD play important roles in scavenging late massive ROS in plant-pathogen interaction. Malonaldehyde (MDA) is widely recognized as an indicator of lipid peroxidation. Membrane damage in plants can be estimated by measuring leakage of electrolytes, which is evaluated by determining relative electrolyte leakage (REL). Less H2O2 and O-2 (-), higher activities of POD and SOD, less MDA content and REL, and higher chlorophyll content and photosynthetic rate were also shown in transgenic plants after inoculation with P. infestans. Our results constitute the first step towards further investigations into the biological function and molecular mechanism of miR172-mediated silencing of AP2/ERF transcription factors in S. lycopersicum-P. infestans interaction and provide a candidate gene for breeding to enhance biotic stress-resistance in S. lycopersicum.
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